UC College of Medicine, Department of Medical Education

Faculty and Staff

Randal E. Morris, PhD
Research Professor Emeritus
Department of Medical Education, University of Cincinnati College of Medicine
(513) 558-6007
randal.morris@uc.edu

Interest:

My research interest over the past 20 years has focused on endocytosis, i.e. the binding, internalization, and intracellular routing of ligands by eucaryotic cells. To monitor these events my colleagues and I have employed a variety of techniques, both biochemical and morphological. My forte is electron microscopy; more specifically, I use immunohistochemical techniques at the ultrastructural level toanswer questions central to cell biology.One of the 2 systems in which I am interested is the interaction of bacterial toxins with mammalian cells. Although I have studied a variety of toxins (including diphtheria, cholera, and botulinum toxins), the greatest effort has focused on the interaction of Pseudomonas exotoxin A (PE) with a sensitive cell line, mouse LM fibroblasts. My colleagues and I have elucidated the binding, internalization, intracellular trafficking of PE by mouse LM cells. Currently we are attempting to determine where activation of the toxin occurs, and from which intracellular compartment the toxin gains entry into the cytosol. Based on kinetic studies and morphological data, my interpretation is that the Golgi is the site of escape. The site of activation is less clear but recent data suggest it is either the cell surface, endosomal compartment, or in the Golgi.The second system to which I devote my research effort is the interaction of the major surfactant protein (SP-A) with rat alveolar Type II epithelial cells. Pulmonary surfactant is a complex mixture of lipids and proteins, of which SP-A is the most abundant protein. Several pathological conditions are associated with surfactant, and these are of significant consequence. Included among these pathological conditions are respiratory disease syndrome of children (hyaline membrane disease), adult respiratory disease syndrome (ARDS), and alveolar proteinosis. The goal of this project is to characterize the autocrine function of SP-A.

Education:

B.S.: Aurora College, 1969
M.S.: Northern Illinois University, 1971
Ph.D.: Emory University, 1974

Awards and Honors:

Most Outstanding Teaching Award-Medical Histology, 2001
Most Outstanding Teaching Award-Medical Histology, 1997
Most Outstanding Teaching Award-Medical Histology, 1993
Most Outstanding Teaching Award-Medical Histology, 1990

Publications

Peer Reviewed Publications (in chronological order):

Newman, S. L., Gootee, L., Hilty, J., & Morris, R. E. (2006). (In Press). Human macrophages do not require phagosome acidification to mediate fungistatic/fungicidal activity against Histoplasma capsulatum. . Journal of Immunology (Baltimore, Md. : 1950) , 176 (3) , 1806-13.

Smith, J. W., Koshoffer, A., Morris, R. E., & Boissy, R. E. (2005). (In Press). Membranous complexes characteristic of melanocytes derived from patients with Hermansky-Pudlak syndrome type 1 are macroautophagosomal entities of the lysosomal compartment. . Pigment Cell Research / Sponsored by the European Society For Pigment Cell Research and the International Pigment Cell Society , 18 (6) , 417-26.

Gildea, L. A., Ciraolo, G. M., Morris, R. E., & Newman, S. L. (2005). (In Press). Human dendritic cell activity against Histoplasma capsulatum is mediated via phagolysosomal fusion. . Infection and Immunity , 73 (10) , 6803-11.

Newman, S. L., Bhugra, B., Holly, A., & Morris, R. E. (2005). (In Press). Enhanced killing of Candida albicans by human macrophages adherent to type 1 collagen matrices via induction of phagolysosomal fusion. . Infection and Immunity , 73 (2) , 770-7.

Jubinsky, P. T., Short, M. K., Mutema, G., Morris, R. E., Ciraolo, G. M., & Li, M. (2005). (In Press). Magmas expression in neoplastic human prostate. . Journal of Molecular Histology , 36 (1-2) , 69-75.

Belecky-Adams, T., Holmes, M., Shan, Y., Tedesco, C. S., Mascari, C., Kaul, A., Wight, D. C., Morris, R. E., Sussman, M., Diamond, J., & Parysek, L. M. (2003). (In Press). An intact intermediate filament network is required for collateral sprouting of small diameter nerve fibers. . The Journal of Neuroscience : the Official Journal of the Society For Neuroscience , 23 (28) , 9312-9.

Long, K. H., Gomez, F. J., Morris, R. E., & Newman, S. L. (2003). (In Press). Identification of heat shock protein 60 as the ligand on Histoplasma capsulatum that mediates binding to CD18 receptors on human macrophages. . Journal of Immunology (Baltimore, Md. : 1950) , 170 (1) , 487-94.

Jubinsky, P. T., Messer, A., Bender, J., Morris, R. E., Ciraolo, G. M., Witte, D. P., Hawley, R. G., & Short, M. K. (2001). (In Press). Identification and characterization of Magmas, a novel mitochondria-associated protein involved in granulocyte-macrophage colony-stimulating factor signal transduction. . Experimental Hematology , 29 (12) , 1392-402.

Sherry, A. M., Malinowska, D. H., Morris, R. E., Ciraolo, G. M., & Cuppoletti, J. (2001). (In Press). Localization of ClC-2 Cl- channels in rabbit gastric mucosa. . American Journal of Physiology. Cell Physiology , 280 (6) , C1599-606.

Gildea, L. A., Morris, R. E., & Newman, S. L. (2001). (In Press). Histoplasma capsulatum yeasts are phagocytosed via very late antigen-5, killed, and processed for antigen presentation by human dendritic cells. . Journal of Immunology (Baltimore, Md. : 1950) , 166 (2) , 1049-56.

Howell, M. L., Alsabbagh, E., Ma, J. F., Ochsner, U. A., Klotz, M. G., Beveridge, T. J., Blumenthal, K. M., Niederhoffer, E. C., Morris, R. E., Needham, D., Dean, G. E., Wani, M. A., & Hassett, D. J. (2000). (In Press). AnkB, a periplasmic ankyrin-like protein in Pseudomonas aeruginosa, is required for optimal catalase B (KatB) activity and resistance to hydrogen peroxide. . Journal of Bacteriology , 182 (16) , 4545-56.

Ochoa, A. L., Mitchner, N. A., Paynter, C. D., Morris, R. E., & Ben-Jonathan, N. (2000). (In Press). Vascular endothelial growth factor in the rat pituitary: differential distribution and regulation by estrogen. . The Journal of Endocrinology , 165 (2) , 483-92.

Morris, R E (2000). (In Press). The use of nonparametric statistics in quantitative electron microscopy. . Journal of Electron Microscopy , 49 (4) , 545-9.

Elhalwagi, B. M., Zhang, M., Ikegami, M., Iwamoto, H. S., Morris, R. E., Miller, M. L., Dienger, K., & McCormack, F. X. (1999). (In Press). Normal surfactant pool sizes and inhibition-resistant surfactant from mice that overexpress surfactant protein A. . American Journal of Respiratory Cell and Molecular Biology , 21 (3) , 380-7.

Strasser, J. E., Newman, S. L., Ciraolo, G. M., Morris, R. E., Howell, M. L., & Dean, G. E. (1999). (In Press). Regulation of the macrophage vacuolar ATPase and phagosome-lysosome fusion by Histoplasma capsulatum. . Journal of Immunology (Baltimore, Md. : 1950) , 162 (10) , 6148-54.

Broomall, K. R., Morris, R. E., Walzer, P. D., & Smulian, A. G. (In Press). Zymolyase treatment exposes p55 antigen of Pneumocystis carinii. . The Journal of Eukaryotic Microbiology , 45 (3) , 284-9.

Rodeberg, D. A., Morris, R. E., & Babcock, G. F. (1997). (In Press). Azurophilic granules of human neutrophils contain CD14. . Infection and Immunity , 65 (11) , 4747-53.

Morris, R. E., & Ciraolo, G. M. (1997). (In Press). A universal post-embedding protocol for immunogold labelling of osmium-fixed, epoxy resin-embedded tissue. . Journal of Electron Microscopy , 46 (4) , 315-9.

Korfhagen, T. R., Bruno, M. D., Ross, G. F., Huelsman, K. M., Ikegami, M., Jobe, A. H., Wert, S. E., Stripp, B. R., Morris, R. E., Glasser, S. W., Bachurski, C. J., Iwamoto, H. S., & Whitsett, J. A. (1996). (In Press). Altered surfactant function and structure in SP-A gene targeted mice. . Proceedings of the National Academy of Sciences of the United States of America , 93 (18) , 9594-9.

Gao, K., Morris, R. E., & Wei, C. (1995). (In Press). Epipolarization microscopic immunogold assay: a combination of immunogold silver staining, enzyme-linked-immunosorbent assay and epipolarization microscopy. . Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission , 70 (4) , 211-6.

Ross, G. F., Morris, R. E., Ciraolo, G., Huelsman, K., Bruno, M., Whitsett, J. A., Baatz, J. E., & Korfhagen, T. R. (1995). (In Press). Surfactant protein A-polylysine conjugates for delivery of DNA to airway cells in culture. . Human Gene Therapy , 6 (1) , 31-40.

Ikeda, K., Clark, J. C., Bachurski, C. J., Wikenheiser, K. A., Cuppoletti, J., Mohanti, S., Morris, R. E., & Whitsett, J. A. (1994). (In Press). Immortalization of subpopulations of respiratory epithelial cells from transgenic mice bearing SV40 large T antigen. . The American Journal of Physiology , 267 (3 Pt 1) , L309-17.

Gao, K., Giffin, B. F., Morris, R. E., Cardell, E. L., & Cardell, R. R. (1994). (In Press). Optimal visualization of immunogold-silver staining of hepatic PEPCK with epipolarized light microscopy. . The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society , 42 (6) , 823-6.

Saelinger, C. B., & Morris, R. E. (1994). (In Press). Uptake and processing of toxins by mammalian cells. . Methods in Enzymology , 235 , 705-17.

Giffin, B. F., Drake, R. L., Morris, R. E., & Cardell, R. R. (1993). (In Press). Hepatic lobular patterns of phosphoenolpyruvate carboxykinase, glycogen synthase, and glycogen phosphorylase in fasted and fed rats. . The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society , 41 (12) , 1849-62.

Giffin, B. F., Gao, K., Morris, R. E., & Cardell, R. R. (1993). (In Press). Enhancement of antigenic site detection with gold labeled secondary and tertiary antibodies using the immunogold-silver staining method. . Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission , 68 (6) , 309-15.

Gao, K., Morris, R. E., Giffin, B. F., & Cardell, R. R. (1993). (In Press). Immunogold-silver staining and epipolarized light microscopic detection of phosphoenolpyruvate carboxykinase and glycogen phosphorylase in rat liver. . Histochemistry , 99 (5) , 341-6.

Kaneshiro, E. S., Reuter, S. F., Quattrone, F. J., & Morris, R. E. (In Press). Sustained food vacuole formation by axenic Paramecium tetraurelia and the inhibition of membrane recycling by Alcian Blue. . The Journal of Protozoology , 39 (6) , 713-8.

Kounnas, M. Z., Morris, R. E., Thompson, M. R., FitzGerald, D. J., Strickland, D. K., & Saelinger, C. B. (1992). (In Press). The alpha 2-macroglobulin receptor/low density lipoprotein receptor-related protein binds and internalizes Pseudomonas exotoxin A. . The Journal of Biological Chemistry , 267 (18) , 12420-3.

Morris, R. E., Ciraolo, G. M., & Saelinger, C. B. (1992). (In Press). Validation of the biotinyl ligand-avidin-gold technique. . The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society , 40 (5) , 711-21.

Thorkelsson, T., Ciraolo, G. M., Ross, G. F., Whitsett, J. A., & Morris, R. E. (1992). (In Press). Lectin activity of the major surfactant protein (SP-A) may participate in, but is not required for, binding to rat type II cells. . The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society , 40 (5) , 643-9.

Morris, R. E., Ciraolo, G. M., & Saelinger, C. B. (1991). (In Press). Gold enhancement of gold-labeled probes: gold-intensified staining technique (GIST). . The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society , 39 (11) , 1585-91.

Forristal, J. J., Thompson, M. R., Morris, R. E., & Saelinger, C. B. (1991). (In Press). Mouse liver contains a Pseudomonas aeruginosa exotoxin A-binding protein. . Infection and Immunity , 59 (9) , 2880-4.

Thompson, M. R., Forristal, J., Kauffmann, P., Madden, T., Kozak, K., Morris, R. E., & Saelinger, C. B. (1991). (In Press). Isolation and characterization of Pseudomonas aeruginosa exotoxin A binding glycoprotein from mouse LM cells. . The Journal of Biological Chemistry , 266 (4) , 2390-6.

Morris, R. E., & Saelinger, C. B. (1990). (In Press). Visualization of intracellular trafficking of proteins. . Methods in Enzymology , 184 , 379-88.

Ryan, R. M., Morris, R. E., Rice, W. R., Ciraolo, G., & Whitsett, J. A. (1989). (In Press). Binding and uptake of pulmonary surfactant protein (SP-A) by pulmonary type II epithelial cells. . The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society , 37 (4) , 429-40.

O'Reilly, M. A., Gazdar, A. F., Morris, R. E., & Whitsett, J. A. (1988). (In Press). Differential effects of glucocorticoid on expression of surfactant proteins in a human lung adenocarcinoma cell line. . Biochimica Et Biophysica Acta , 970 (2) , 194-204.

Saelinger, C. B., & Morris, R. E. (1987). (In Press). Intracellular trafficking of Pseudomonas exotoxin A. . Antibiotics and Chemotherapy , 39 , 149-59.

Morris, R. E., & Saelinger, C. B. (1986). (In Press). Problems in the production and use of 5 nm avidin-gold colloids. . Journal of Microscopy , 143 (Pt 2) , 171-6.

Morris, R. E., & Saelinger, C. B. (1986). (In Press). Reduced temperature alters Pseudomonas exotoxin A entry into the mouse LM cell. . Infection and Immunity , 52 (2) , 445-53.

Morris, R. E., Gerstein, A. S., Bonventre, P. F., & Saelinger, C. B. (1985). (In Press). Receptor-mediated entry of diphtheria toxin into monkey kidney (Vero) cells: electron microscopic evaluation. . Infection and Immunity , 50 (3) , 721-7.

Saelinger, C. B., Morris, R. E., & Foertsch, G. (1985). (In Press). Trafficking of Pseudomonas exotoxin A in mammalian cells. . European Journal of Clinical Microbiology , 4 (2) , 170-4.

Morris, R. E., & Saelinger, C. B. (1985). (In Press). Route of pseudomonas and diphtheria toxin entry into mammalian cells: basis for susceptibility to toxin. . Survey and Synthesis of Pathology Research , 4 (1) , 34-43.

Manhart, M. D., Morris, R. E., Bonventre, P. F., Leppla, S., & Saelinger, C. B. (1984). (In Press). Evidence for pseudomonas exotoxin A receptors on plasma membrane of toxin-sensitive lm fibroblasts. . Infection and Immunity , 45 (3) , 596-603.

Cohen, D. A., Morris, R. E., & Bubel, H. C. (1984). (In Press). Abortive ectromelia virus infection in peritoneal macrophages activated by Corynebacterium parvum. . Journal of Leukocyte Biology , 35 (2) , 179-92.

Morris, R. E., & Saelinger, C. B. (1984). (In Press). Visualization of intracellular trafficking: use of biotinylated ligands in conjunction with avidin-gold colloids. . The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society , 32 (1) , 124-8.

Morris, R. E., & Saelinger, C. B. (1983). (In Press). Diphtheria toxin does not enter resistant cells by receptor-mediated endocytosis. . Infection and Immunity , 42 (2) , 812-7.

FitzGerald, D., Morris, R. E., & Saelinger, C. B. (In Press). Inhibition of the activity of pseudomonas toxin by methylamine. . Reviews of Infectious Diseases , 5 Suppl 5 , S985-91.

Bentley, D. M., Watson, S. R., & Morris, R. E. (1983). (In Press). Age-related loss of Lyt-1,2 cells in C58 mice results in susceptibility to lactic dehydrogenase virus-induced polioencephalomyelitis. . Infection and Immunity , 41 (3) , 1389-90.

Barnett, J. H., Estes, S. A., Wirman, J. A., Morris, R. E., & Staneck, J. L. (1983). (In Press). Erysipeloid. . Journal of the American Academy of Dermatology , 9 (1) , 116-23.

Morris, R. E., Manhart, M. D., & Saelinger, C. B. (1983). (In Press). Receptor-mediated entry of Pseudomonas toxin: methylamine blocks clustering step. . Infection and Immunity , 40 (2) , 806-11.

Bentley, D. M., Guckian, V. A., Stinnett, J. D., & Morris, R. E. (1982). (In Press). Failure to demonstrate a role for line Ib tumor-associated surface antigen in the etiology of age-dependent polioencephalomyelitis. . Molecular Immunology , 19 (8) , 983-9.

Morris, R. E., David, C. S., & Manhart, M. D. (1982). (In Press). Tumor and histocompatibility antigen interaction: direct visualization by a double-label bridging technique for immuno-electron microscopy. . Molecular Immunology , 19 (8) , 973-82.

Morris, R. E., & Saelinger, C. B. (1982). (In Press). Combined use of immunoferritin and immunocolloidal gold for the simultaneous demonstration of two antigens by immuno-electron microscopy. . Stain Technology , 57 (4) , 225-37.

Morris, R. E., & Saelinger, C. B. (1982). (In Press). A simple reliable method for producing electron dense markers of uniform size for use in immunoelectron microscopy. . Journal of Immunological Methods , 49 (3) , 237-46.

Fitzgerald, D., Morris, R. E., & Saelinger, C. B. (1982). (In Press). Essential role of calcium in cellular internalization of Pseudomonas toxin. . Infection and Immunity , 35 (2) , 715-20.

Bentley, D. M., & Morris, R. E. (1982). (In Press). T cell subsets required for protection against age-dependent polioencephalomyelitis of C58 mice. . Journal of Immunology (Baltimore, Md. : 1950) , 128 (2) , 530-4.

Mbuy, G. N., Morris, R. E., & Bubel, H. C. (1982). (In Press). Inhibition of cellular protein synthesis by vaccinia virus surface tubules. . Virology , 116 (1) , 137-47.

Gelman, B. B., Davis, M. H., Morris, R. E., & Gruenstein, E. (1981). (In Press). Structural changes in lysosomes from cultured human fibroblasts in Duchenne's muscular dystrophy. . The Journal of Cell Biology , 88 (2) , 329-37.

Morris, R. E., Bentley, D. M., & Ciraolo, G. M. (1981). (In Press). Quantitation of tumor antigen expression on the surface of malignant line Ib lymphocytes by immuno-electron microscopy. . Journal of the National Cancer Institute , 66 (1) , 111-9.

FitzGerald, D., Morris, R. E., & Saelinger, C. B. (1980). (In Press). Receptor-mediated internalization of Pseudomonas toxin by mouse fibroblasts. . Cell , 21 (3) , 867-73.

Morris, R. E., Ciraolo, G. M., Cohen, D. A., & Bubel, H. C. (1980). (In Press). In situ fixation of cultured mouse peritoneal exudate cells: comparison of fixation methods. . In Vitro , 16 (2) , 136-46.

Hale, T. L., Morris, R. E., & Bonventre, P. F. (1979). (In Press). Shigella infection of henle intestinal epithelial cells: role of the host cell. . Infection and Immunity , 24 (3) , 887-94.

Morris, R. E., & Murphy, W. H. (1978). (In Press). Immunogenicity and paralytogenicity of antigen solubilized from line Ib malignant lymphocytes. . Proceedings of the Society For Experimental Biology and Medicine. Society For Experimental Biology and Medicine (New York, N.Y.) , 157 (2) , 330-5.

Morris, R. E., & Fritz, R. B. (1976). (In Press). Expression of virion and tumor-specific antigens on the surface of chick embryo cells infected with strain MC29 avian leukosis virus. . Cancer Research , 36 (2 Pt 1) , 414-9.

Morris, R. E., & Fritz, R. B. (1975). (In Press). Ultrastructural visualization of virus-induced surface antigens. Comparative studies of three immunohistochemical techniques. . The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society , 23 (11) , 855-62.